A Plasmid Construct for Testing the Resistance of HIV-1 Subtype C Virus
The object of this technology is to propose a plasmid containing the genome of Human Immunodeficiency Virus type 1 (HIV-1) for effectual testing of effectiveness of chemical compounds to inhibit HIV-1 replication in-vitro. The technology suggest recombinant plasmid pPS4.3HSAR-E; comprising HIV-1 genome with defective envelope gene and mutated gag-pol gene wherein Xmal and SacII restriction enzyme sites have been introduced on either side of reverse transcriptase (RT) gene inserted in a plasmid vector. It also suggest that plasmid containing genome of HIV-1 from which RT gene can be easily removed and replaced with RT of any other HIV-1 isolate. It also offers the method for constructing the plasmid for effective testing. The viral genome is mounted onto a carrier DNA molecule called a plasmid. This plasmid when transfected in an eukaryotic cell expresses the proteins of virus on the surface of the cell which can be easily visualized either under the immunofluorescent microscope or by flowcytometer or Enzyme Linked Immunosorbent Assay (ELISA). The expression of viral proteins on the cell surface is inhibited by antiretrovirals.
Sector: Medical Technologies
Area of Application: Medical and diagnostic industry.
Keywords: HIV-1 Subtype C virus
Advantages: Safer to use Less labour intensive
Environmental aspects: Not Applicable
Development Status: Laboratory Model
Legal Protection: Patent
Transfer Terms: Technology Licensing
Target Countries: India
Estimated cost (US$):
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