A Rapid Diagnostic Kit and Reagents for Detecting Dengue Infections
The technology relates to novel kit and reagents for diagnosis of Dengue viral infections. The kit relates to diagnosis of the four known closely related, antigenically distinct serotypes of Dengue virus. More particularly, the technology relates to multiepitope recombinant proteins and their use in the diagnosis of dengue and other viral infections. The technology provides two dengue multiepitope proteins, one designed to detect IgM (Immunoglobin M) and the other to detect IgG (Immunoglobin G) antibodies in dengue patient sera. An important aspect of the technology resides in designing and expressing two novel recombinant protein antigens by assembling key immunodominant linear dengue-specific epitopes, chosen on the basis of pepscan analysis, phage display and computer predictions. One of these developed to specifically detect dengue-specific IgM and the other to detect IgG These novel recombinant dengue multiepitope proteins were expressed in E. coli, purified in a single step and used as capture antigens in ELISA. The ELISA results, using a large panel of suspected dengue patient sera, were in excellent agreement with those obtained using the commercially available Dengue Duo IgM and IgG Rapid strip test (PanBio). The technology also provides a simple and rapid spot test for dengue detection using these recombinant multiepitope proteins. The high epitope density, careful choice of epitopes and the use of E. coli system for expression has resulted in inexpensive diagnostic tests kits and methods with a high degree of sensitivity and specificity.
Sector: Medical Technologies
Area of Application: Diagnostic Kit and Reagents for Detecting Dengue Infections
Keywords: dengue, diagnostic kit
Advantages: Kits are highly specific and sensitive Kits and reagents are simple, rapid and inexpensive Capable of detection both IgM and IgG antibodies Simple rapid spot test for dengue detection
Environmental aspects: Not Applicable
Development Status: Laboratory Model
Legal Protection: Patent in Progress
Transfer Terms: Technology Licensing
Estimated cost (US$):
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Contact Person: SkyQuest Technology Consulting Pvt. Ltd.
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