Detection of Causative Agents of Ocular and Nervous System Infections
The present technology is related to diagnosis method for detection of single or more causative agents of ocular and nervous system infections among many probable pathogens. The invention provides a rapid assay for the simultaneous detection of the pathogens responsible for infections of the eye and central nervous system for which immunological parameters are not indicative of an active infection but only indicative of exposure to the pathogen and for which classical microbiological assays such as bacterial and fungal cultures are neither sensitive enough to detect the pathogen nor rapid enough to identify the pathogen within 48 hours. In addition aqueous humor or vitreous fluid in case of endophthalmitis will be sufficient in order to detect and discriminate the infectious agent. This obviates the necessity of surgical procedure such as vitrectomy to be performed in an operating room. This technology combines the high sensitivity of PCR assay and the high specificity of identification by hybridization on to a macro-array with the detection of hybridization by color detection methods, the end result of which can be monitored by naked eye. The invention features a multiplex assay for the simultaneous detection and discrimination of pathogens that cause infections of the eye and CNS comprising: 1) Processing the clinical samples such as corneal scraping or conjunctival swab or aqueous humor or vitreous fluid or vitrectomy lavage collected or cerebrospinal fluid aspirate or pus collected from brain abscess material or an epiretinal membrane from a patient suspected of being afflicted with eye or a CNS infection, to isolate DNA by standard methods. 2) Amplifying a specific region of DNA from a gene that is specific to each of the pathogens by a single tube PCR technique using labeled amplification primers for each of the pathogens known to cause CNS and eye infections. 3) Detection and discrimination of pathogens using a DNA hybridization wherein the immobilized target sequences specific for each of the pathogens are reacted with amplified DNA probes generated from the PCR reaction and monitoring the hybridization by color development using specific set of reagents.
Area of Application: Medical industry
Advantages: Huge market opportunity. It helps the physician to select the appropriate treatment. Highly efficient and time saving kit. Useful for a treatment using combination of drugs for effective therapy. Identification of specific pathogen at very early stage of infection. Effective in Identification of multiple infection from same sample.
Environmental aspects: Not Applicable
Development Status: Laboratory Model
Legal Protection: Patent in Progress
Transfer Terms: Technology Licensing
Target Countries: India
Estimated cost (US$):
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