Extracellular Phytase with Improved Thermostability
The proposed technology relates to a process for the production of extracellular phytase using a novel isolate of fungi. The phytases so produced using this process are thermostable and acid stable. The process comprises: i) isolating phytase producing microorganisms from soil and water samples taken from live-stock units, poultry farms and rotten wood logs, identified and characterized the novel strain. ii) keeping the strain in the phytase screening agar medium (PSM) to obtain culture and incubating the above the culture at 30°C to 50°C for 24 to 48 hours. Inoculating the microorganism in PSM broth, starch medium, Luria broth and minimal medium to produce phytase, recovering phytase from the fermentation broth by conventional centrifugation and filtration method.
Sector: Medical Technologies
Area of Application: Food industry, pharma industry
Keywords: thermostability,phosphorus pollution
Advantages: Available wild type strain is producing phytase which is at par with known recombinant strains Enzyme produced is highly thermostable, with no loss of activity at 55°C even after 24 hours of incubation. Enzyme produced possesses broad substrate specificity and can hydrolyse phosphate groups from variety of phospho-monoesters, thus making it a potential candidate in feed applications. Enzyme produced is very much stable under acidic conditions i.e., no significant loss of activity even after 45 days at 4°C, pH 2.5.
Environmental aspects: Not Applicable
Development Status: Laboratory Model
Legal Protection: Patent
Transfer Terms: Technology Licensing
Target Countries: India
Estimated cost (US$):
Upload any relevant document:
Contact Person: SkyQuest Technology Consulting Pvt. Ltd.
Address: 501, Krishna Complex, Opp. Devashish School, Bodakdev
Zip/Pin Code: 380054